A Mismatch-tolerant RT-LAMP Method for Molecular Diagnosis of Highly Variable Viruses
نویسندگان
چکیده
منابع مشابه
Development of Molecular Diagnosis Method for Identification of Hcv Using Lamp and Rt-pcr Techniques
Hepatitis C is blood-borne viral disease worldwide. Chronic hepatitis C infection leads to cirrhosis of the liver, liver failure and liver cancer. Antiviral therapy exists and approximately 50% of infected individuals can be cured. The importance of low cost molecular diagnostic assays are especially important for the developing nations as they are already burdened with increasing number of hep...
متن کاملComparison between RT-PCR, NASBA and RT-LAMP Methods for Detection of Coxsackievirus B3
Viral myocarditis is a moderate disease, but it sometimes causes progressive cardiac disorder. Many different viruses have been considered as the agent of viral myocarditis, but Coxsackievirus of the B group, in particular of the Coxsackievirus B3 (CVB3), is more than fifty percent of cases of viral myocarditis. CVB3 is a positive single-stranded RNA virus and a member of the genus Enterovirus ...
متن کاملMiniature RT–PCR system for diagnosis of RNA-based viruses
This paper presents an innovative portable chip-based RT-PCR system for amplification of specific nucleic acid and detection of RNA-based viruses. The miniature RT-PCR chip is fabricated using MEMS (Micro-electro-mechanical-system) techniques, and comprises a micro temperature control module and a PDMS (polydimethylsiloxane)-based microfluidic control module. The heating and sensing elements of...
متن کاملRapid Detection of COVID-19 by RT-LAMP PCR Technique and its Comparison with Real-Time RT-PCR Method
Rapid antigen and antibody, serological tests, and RT ‑ PCR-based molecular methods are widely used for the detection of microorganisms worldwide. Objectives: This study aimed by Isothermal nucleic acid amplification techniques to detect the covid-19. Methods: In this study, 200 samples of nasopharynx and oropharynx were collected from Jamaran Heart Hospital in Tehran. Covid -19 was exami...
متن کاملMolecular Diagnosis of Plant Viruses
Makkouk, K.M. and S.G. Kumari. 2006. Molecular Diagnosis of Plant Viruses. Arab J. Pl. Prot. 24: 135-138. Proper virus identification is always the key in developing appropriate practical solutions to manage plant virus diseases. Recent advances in biotechnology and molecular biology have played a significant role in the development of rapid, specific and sensitive diagnostic tests. The use of ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BIO-PROTOCOL
سال: 2019
ISSN: 2331-8325
DOI: 10.21769/bioprotoc.3415